Drought-Induced Morpho-Physiological, Biochemical, Metabolite Responses and Protein Profiling of Chickpea (Cicer arietinum L.)

The chickpea (Cicer arieitnum L.) is an important food legume crop of the family Fabaceae with high protein levels that widely grown in rainfed areas prone to drought stress. It a self-pollinated cool season true diploid (2n = 16) nature. relatively cheap and source protein. About 90% by use residual moisture soil without depending on irrigation. In present study, two varieties chickpea, namely ICC 4958 HC-6, were under three field capacities (FC) (100% FC, 50% FC 25% FC). Samples taken times, i.e., 15, 30 45 days after sowing (DAS). Parameters such as morphological, physiological, biochemical, metabolite profiling completed. Morphological parameters shoot length (14.2%), number branches (20.7%), leaves (17.5%) yield (56%) declined level increased other characteristics root (9.7%), flowers (24.5%) pods (34.4%) stress progressed. Physiological relative water content (RWC) (13.5%), cell membrane stability (CMS) (29.6%) chlorophyll decreased, whereas electrolyte conductivity (EC) (38%) both capacity decreased. Biochemical proline (54.75%), sugar (15.2%), glycine betaine (32.25%), superoxide dismutase (SOD) (49.5%), catalase (CAT) (50.5%), ascorbate peroxidase (APX) (44.9%) glutathione reductase (GR) (49%) increased. Metabolite analyses of, for example, MDA (malondialdehyde) (30.5%), total anthocyanin (36.3%), flavonoid (26%) phenolic (29.5%) We also performed using SDS-PAGE (sodium dodecyl-sulfate polyacrylamide gel electrophoresis) differentiate expression analysis varieties.